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But Twitter does not actually get to see the money.
Andreessen Horowitz made the purchase through the private secondary markets, which connects early investors and Twitter employee able to sell privately held shares with outside investors.
The buy in equates to a 2 percent ownership share of Twitter and comes on the heels of the company securing $200 million of funding in December.
Andreessen Horowitz was not part of that funding round. The venture capital firm now owns shares in Zynga, Groupon, Foursquare and Twitter.